FREQUENTLY ASKED QUESTIONS

 

This part of the homepage should answer you some questions, our customer support gets asked very often. If you can’t find answers to your questions in this list or think there should be one question added, please contact This email address is being protected from spambots. You need JavaScript enabled to view it. .

 

We tried to make our products as self explaining and fail proof as possible but sometimes questions occur:

 

Wash buffer, sample buffer, substrate, stop solution.Never mix standards or conjugate!

Wash buffer, sample buffer, substrate, stop solution. Never mix standards or conjugate!

The stability of the various components is different. The expiry date for the whole kit is determined by the component with the lowest shelf life, that’s usually the conjugate.

Yes, for the wash solution, sample buffer, substrate and stop. Please give us the reference and lot number.

Yes, all kinds of plasma can be used just like serum.

No. Our stability tests prove sufficient stability even at 56°C. However, the kits should be stored at 2-8°C upon receipt.

NO ! Never freeze the kits or any component out of it. Freezing will destroy the reagents and the coating of the plate.

No. Only a six-point-calibration gives exact results. We do not guarantee for results made with fewer than six standards.

OD values in the QC certificate have been measured under standardized conditions in our QC lab. Immunological and enzymatic reactions are highly temperature dependent, thus the differences between different labs.

The majority of autoimmune ELISA tests are not standardized and there are significant differences between the antigen quality/source. In those comparisons, the “gold standard” should always be the clinical data of the patients.

We have introduced a grey zone in the Automatic line due to the inter- and intra- lab variability caused by the temperature fluctuations on the different automated systems. The grey zone allows a clear and easier discrimination of the results around the cut-off value, especially when using automated systems.

No. The cut-off calibrator value still being 15 U/ml. The correct equivocal range was determined by testing 384 samples of healthy blood donors and it was calculated as a sum of the mean value plus 3 x standard deviation. The lower limit (12 U/ml) resulted from the 95% percentile and the upper limit (18 U/ml) from the 99% percentile.

 

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